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kaleidoscope polypeptide standard molecular mass pattern  (Bio-Rad)


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    Bio-Rad kaleidoscope polypeptide standard molecular mass pattern
    Kaleidoscope Polypeptide Standard Molecular Mass Pattern, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kaleidoscope+polypeptide+standards/kaleidoscope+polypeptide+standard+molecular+mass+pattern/10__1080_slash_10498850__2015__1080775-73-51-59
    Average 90 stars, based on 1 article reviews
    kaleidoscope polypeptide standard molecular mass pattern - by Bioz Stars, 2026-10
    90/100 stars

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    Molecular Weight:

    Article Title: Variation in lethality and effects of two Australian chirodropid jellyfish venoms in fish.
    Article Snippet: The North Queensland chirodropid box jellyfish Chironex fleckeri and Chiropsalmus sp. share similar nematocyst composition and the same prey of Acetes australis shrimps in their early medusa stages; however, as C. fleckeri individuals reach larger size, the animals add fish to their diet and their complement of nematocyst types changes, allowing larger doses of venom to be delivered to prey.. This study demonstrated that the venoms of the two species differ as well: despite similar effects previously documented in crustacean prey models, the two had widely different cardiac and lethal effects in fish, with C. fleckeri being substantially more potent in its ability to cause death.. Comparisons between the venom delivery abilities of the two species showed that the change in nematocysts of C. fleckeri cannot alone account for its ontogenetic shift to prey fish; instead, its prey ecology clearly necessitates it having venom capable of acting efficiently to cause death in fish.

    Article Title: Molecular Cloning and Characterization of First Organic Matrix Protein from Sclerites of Red Coral, Corallium rubrum
    Article Snippet: Bis-Tris 12% polyacrylamide gels (Criterion, Biorad) were used for wide range molecular weight protein; and TrisTricine 16.5% poly-acrylamide gels (Criterion, Biorad) for small molecular weight proteins. .. The protein markers used were: Silver Stain Molecular Weight Marker (Sigma M6539) for the Bis-Tris at U niversity of Illinois at C hicago Library, on M ay 8, 2012 w w w .jbc.org D ow nloaded from 3 silver stained electrophoresis gel, Kaleidoscope Polypeptide Standards (Biorad 161-0325) for the Tris-Tricine silver stained electrophoresis gels, and Precision Plus Protein WesternCTM standard (Biorad 161-0376) both for the Bis-Tris and TrisTricine gels of Western blots. .. The primary antibodies used for Western blots were anti-phospho Serine (Abcam 9332), antiphospho Threonine (Abcam 9337), anti-phospho Tyrosine (Abcam 9319) and polyclonal antibodies raised against two amino acid sequences of the organic matrix (OM) protein identified in the present study i.e. scleritin: NH2FIELSKRMQRESSNFC-CONH2 and NH2CNTRPVQPISRQLDDL-CONH2.

    Article Title: Molecular Cloning and Characterization of First Organic Matrix Protein from Sclerites of Red Coral, Corallium rubrum
    Article Snippet: BisTris 12% polyacrylamide gels (Criterion, Bio-Rad) were used for wide range molecular weight protein; and Tris-Tricine 16.5% polyacrylamide gels (Criterion, Bio-Rad) for small molecular weight proteins. .. The protein markers used were: Silver Stain Molecular Weight Marker (M6539; Sigma) for the BisTris silver-stained electrophoresis gel, Kaleidoscope Polypeptide Standards (Bio-Rad 161-0325) for the Tris-Tricine silver-stained electrophoresis gels, and Precision Plus Protein WesternC TM standard (161-0376 Bio-Rad) both for the BisTris and Tris-Tricine gels of Western blots. .. The primary antibodies used for Western blotting were anti-phosphoserine (9332; Abcam), anti-phosphothreonine (9337; Abcam), anti-phosphotyrosine (9319; Abcam), and polyclonal antibodies raised against two amino acid sequences of the OM protein identified in the present study, i.e. scleritin: NH 2 -FIELSKRMQRESSNFC-COOH and NH 2 -CNTRPVQPISRQLDDL-COOH.

    Silver Staining:

    Article Title: Molecular Cloning and Characterization of First Organic Matrix Protein from Sclerites of Red Coral, Corallium rubrum
    Article Snippet: Bis-Tris 12% polyacrylamide gels (Criterion, Biorad) were used for wide range molecular weight protein; and TrisTricine 16.5% poly-acrylamide gels (Criterion, Biorad) for small molecular weight proteins. .. The protein markers used were: Silver Stain Molecular Weight Marker (Sigma M6539) for the Bis-Tris at U niversity of Illinois at C hicago Library, on M ay 8, 2012 w w w .jbc.org D ow nloaded from 3 silver stained electrophoresis gel, Kaleidoscope Polypeptide Standards (Biorad 161-0325) for the Tris-Tricine silver stained electrophoresis gels, and Precision Plus Protein WesternCTM standard (Biorad 161-0376) both for the Bis-Tris and TrisTricine gels of Western blots. .. The primary antibodies used for Western blots were anti-phospho Serine (Abcam 9332), antiphospho Threonine (Abcam 9337), anti-phospho Tyrosine (Abcam 9319) and polyclonal antibodies raised against two amino acid sequences of the organic matrix (OM) protein identified in the present study i.e. scleritin: NH2FIELSKRMQRESSNFC-CONH2 and NH2CNTRPVQPISRQLDDL-CONH2.

    Article Title: Molecular Cloning and Characterization of First Organic Matrix Protein from Sclerites of Red Coral, Corallium rubrum
    Article Snippet: BisTris 12% polyacrylamide gels (Criterion, Bio-Rad) were used for wide range molecular weight protein; and Tris-Tricine 16.5% polyacrylamide gels (Criterion, Bio-Rad) for small molecular weight proteins. .. The protein markers used were: Silver Stain Molecular Weight Marker (M6539; Sigma) for the BisTris silver-stained electrophoresis gel, Kaleidoscope Polypeptide Standards (Bio-Rad 161-0325) for the Tris-Tricine silver-stained electrophoresis gels, and Precision Plus Protein WesternC TM standard (161-0376 Bio-Rad) both for the BisTris and Tris-Tricine gels of Western blots. .. The primary antibodies used for Western blotting were anti-phosphoserine (9332; Abcam), anti-phosphothreonine (9337; Abcam), anti-phosphotyrosine (9319; Abcam), and polyclonal antibodies raised against two amino acid sequences of the OM protein identified in the present study, i.e. scleritin: NH 2 -FIELSKRMQRESSNFC-COOH and NH 2 -CNTRPVQPISRQLDDL-COOH.

    Marker:

    Article Title: Molecular Cloning and Characterization of First Organic Matrix Protein from Sclerites of Red Coral, Corallium rubrum
    Article Snippet: Bis-Tris 12% polyacrylamide gels (Criterion, Biorad) were used for wide range molecular weight protein; and TrisTricine 16.5% poly-acrylamide gels (Criterion, Biorad) for small molecular weight proteins. .. The protein markers used were: Silver Stain Molecular Weight Marker (Sigma M6539) for the Bis-Tris at U niversity of Illinois at C hicago Library, on M ay 8, 2012 w w w .jbc.org D ow nloaded from 3 silver stained electrophoresis gel, Kaleidoscope Polypeptide Standards (Biorad 161-0325) for the Tris-Tricine silver stained electrophoresis gels, and Precision Plus Protein WesternCTM standard (Biorad 161-0376) both for the Bis-Tris and TrisTricine gels of Western blots. .. The primary antibodies used for Western blots were anti-phospho Serine (Abcam 9332), antiphospho Threonine (Abcam 9337), anti-phospho Tyrosine (Abcam 9319) and polyclonal antibodies raised against two amino acid sequences of the organic matrix (OM) protein identified in the present study i.e. scleritin: NH2FIELSKRMQRESSNFC-CONH2 and NH2CNTRPVQPISRQLDDL-CONH2.

    Article Title: Proteomic scan for tyrosinase peptide antigenic pattern in vitiligo and melanoma: role of sequence similarity and HLA-DR1 affinity.
    Article Snippet: Signals were visualized by ECL reaction (ECL kit; Sigma-Aldrich). .. Kaleidoscope polypeptide standards (catalog 161-0325; Bio-Rad) were used as kilodalton marker. ..

    Article Title: Molecular Cloning and Characterization of First Organic Matrix Protein from Sclerites of Red Coral, Corallium rubrum
    Article Snippet: BisTris 12% polyacrylamide gels (Criterion, Bio-Rad) were used for wide range molecular weight protein; and Tris-Tricine 16.5% polyacrylamide gels (Criterion, Bio-Rad) for small molecular weight proteins. .. The protein markers used were: Silver Stain Molecular Weight Marker (M6539; Sigma) for the BisTris silver-stained electrophoresis gel, Kaleidoscope Polypeptide Standards (Bio-Rad 161-0325) for the Tris-Tricine silver-stained electrophoresis gels, and Precision Plus Protein WesternC TM standard (161-0376 Bio-Rad) both for the BisTris and Tris-Tricine gels of Western blots. .. The primary antibodies used for Western blotting were anti-phosphoserine (9332; Abcam), anti-phosphothreonine (9337; Abcam), anti-phosphotyrosine (9319; Abcam), and polyclonal antibodies raised against two amino acid sequences of the OM protein identified in the present study, i.e. scleritin: NH 2 -FIELSKRMQRESSNFC-COOH and NH 2 -CNTRPVQPISRQLDDL-COOH.

    Staining:

    Article Title: Molecular Cloning and Characterization of First Organic Matrix Protein from Sclerites of Red Coral, Corallium rubrum
    Article Snippet: Bis-Tris 12% polyacrylamide gels (Criterion, Biorad) were used for wide range molecular weight protein; and TrisTricine 16.5% poly-acrylamide gels (Criterion, Biorad) for small molecular weight proteins. .. The protein markers used were: Silver Stain Molecular Weight Marker (Sigma M6539) for the Bis-Tris at U niversity of Illinois at C hicago Library, on M ay 8, 2012 w w w .jbc.org D ow nloaded from 3 silver stained electrophoresis gel, Kaleidoscope Polypeptide Standards (Biorad 161-0325) for the Tris-Tricine silver stained electrophoresis gels, and Precision Plus Protein WesternCTM standard (Biorad 161-0376) both for the Bis-Tris and TrisTricine gels of Western blots. .. The primary antibodies used for Western blots were anti-phospho Serine (Abcam 9332), antiphospho Threonine (Abcam 9337), anti-phospho Tyrosine (Abcam 9319) and polyclonal antibodies raised against two amino acid sequences of the organic matrix (OM) protein identified in the present study i.e. scleritin: NH2FIELSKRMQRESSNFC-CONH2 and NH2CNTRPVQPISRQLDDL-CONH2.

    Electrophoresis:

    Article Title: Molecular Cloning and Characterization of First Organic Matrix Protein from Sclerites of Red Coral, Corallium rubrum
    Article Snippet: Bis-Tris 12% polyacrylamide gels (Criterion, Biorad) were used for wide range molecular weight protein; and TrisTricine 16.5% poly-acrylamide gels (Criterion, Biorad) for small molecular weight proteins. .. The protein markers used were: Silver Stain Molecular Weight Marker (Sigma M6539) for the Bis-Tris at U niversity of Illinois at C hicago Library, on M ay 8, 2012 w w w .jbc.org D ow nloaded from 3 silver stained electrophoresis gel, Kaleidoscope Polypeptide Standards (Biorad 161-0325) for the Tris-Tricine silver stained electrophoresis gels, and Precision Plus Protein WesternCTM standard (Biorad 161-0376) both for the Bis-Tris and TrisTricine gels of Western blots. .. The primary antibodies used for Western blots were anti-phospho Serine (Abcam 9332), antiphospho Threonine (Abcam 9337), anti-phospho Tyrosine (Abcam 9319) and polyclonal antibodies raised against two amino acid sequences of the organic matrix (OM) protein identified in the present study i.e. scleritin: NH2FIELSKRMQRESSNFC-CONH2 and NH2CNTRPVQPISRQLDDL-CONH2.

    Article Title: Molecular Cloning and Characterization of First Organic Matrix Protein from Sclerites of Red Coral, Corallium rubrum
    Article Snippet: BisTris 12% polyacrylamide gels (Criterion, Bio-Rad) were used for wide range molecular weight protein; and Tris-Tricine 16.5% polyacrylamide gels (Criterion, Bio-Rad) for small molecular weight proteins. .. The protein markers used were: Silver Stain Molecular Weight Marker (M6539; Sigma) for the BisTris silver-stained electrophoresis gel, Kaleidoscope Polypeptide Standards (Bio-Rad 161-0325) for the Tris-Tricine silver-stained electrophoresis gels, and Precision Plus Protein WesternC TM standard (161-0376 Bio-Rad) both for the BisTris and Tris-Tricine gels of Western blots. .. The primary antibodies used for Western blotting were anti-phosphoserine (9332; Abcam), anti-phosphothreonine (9337; Abcam), anti-phosphotyrosine (9319; Abcam), and polyclonal antibodies raised against two amino acid sequences of the OM protein identified in the present study, i.e. scleritin: NH 2 -FIELSKRMQRESSNFC-COOH and NH 2 -CNTRPVQPISRQLDDL-COOH.

    Western Blot:

    Article Title: Molecular Cloning and Characterization of First Organic Matrix Protein from Sclerites of Red Coral, Corallium rubrum
    Article Snippet: Bis-Tris 12% polyacrylamide gels (Criterion, Biorad) were used for wide range molecular weight protein; and TrisTricine 16.5% poly-acrylamide gels (Criterion, Biorad) for small molecular weight proteins. .. The protein markers used were: Silver Stain Molecular Weight Marker (Sigma M6539) for the Bis-Tris at U niversity of Illinois at C hicago Library, on M ay 8, 2012 w w w .jbc.org D ow nloaded from 3 silver stained electrophoresis gel, Kaleidoscope Polypeptide Standards (Biorad 161-0325) for the Tris-Tricine silver stained electrophoresis gels, and Precision Plus Protein WesternCTM standard (Biorad 161-0376) both for the Bis-Tris and TrisTricine gels of Western blots. .. The primary antibodies used for Western blots were anti-phospho Serine (Abcam 9332), antiphospho Threonine (Abcam 9337), anti-phospho Tyrosine (Abcam 9319) and polyclonal antibodies raised against two amino acid sequences of the organic matrix (OM) protein identified in the present study i.e. scleritin: NH2FIELSKRMQRESSNFC-CONH2 and NH2CNTRPVQPISRQLDDL-CONH2.

    Article Title: Molecular Cloning and Characterization of First Organic Matrix Protein from Sclerites of Red Coral, Corallium rubrum
    Article Snippet: BisTris 12% polyacrylamide gels (Criterion, Bio-Rad) were used for wide range molecular weight protein; and Tris-Tricine 16.5% polyacrylamide gels (Criterion, Bio-Rad) for small molecular weight proteins. .. The protein markers used were: Silver Stain Molecular Weight Marker (M6539; Sigma) for the BisTris silver-stained electrophoresis gel, Kaleidoscope Polypeptide Standards (Bio-Rad 161-0325) for the Tris-Tricine silver-stained electrophoresis gels, and Precision Plus Protein WesternC TM standard (161-0376 Bio-Rad) both for the BisTris and Tris-Tricine gels of Western blots. .. The primary antibodies used for Western blotting were anti-phosphoserine (9332; Abcam), anti-phosphothreonine (9337; Abcam), anti-phosphotyrosine (9319; Abcam), and polyclonal antibodies raised against two amino acid sequences of the OM protein identified in the present study, i.e. scleritin: NH 2 -FIELSKRMQRESSNFC-COOH and NH 2 -CNTRPVQPISRQLDDL-COOH.

    other:

    Article Title: Angiotensin I-converting enzyme inhibitory properties of Phaseolus vulgaris bean hydrolysates: Effects of different thermal and enzymatic digestion treatments
    Article Snippet: a Food Research and Development Centre, Agriculture and Agri-Food Canada, 3600 Casavant Blvd.. W., St-Hyacinthe, QC, Canada J2S 8E3 b Department of Bioresource Engineering, Macdonald Campus, McGill University, 21,111 Lakeshore Rd., Ste-Anne-de-Bellevue, QC, Canada H9X 3V9 c Department of Food Science and Agricultural Chemistry, Macdonald Campus, McGill University, 21,111 Lakeshore Rd., Ste-Anne-de-Bellevue, QC, Canada H9X 3V9

    Article Title: Expression levels and subcellular localization of Bcy1p in Candida albicans mutant strains devoid of one BCY1 allele results in a defective morphogenetic behavior.
    Article Snippet: Reagents were purchased as follows: kemptide (LRRASLG), cAMP, Calcofluor white, ATP, anti-rabbit IgG and anti-mouse IgG (both conjugated to alkaline phosphatase), 4,6-diamino-2-phenylindol, trypan blue, Sigma Chemical Co.; phosphocellulose paper P-81 and DE-52 were from Whatman; kaleidoscope polypeptide standards, BioRad; polyvinylidene difluoride membranes (Immobilon-P), Millipore Corp.; monoclonal anti-green fluorescent protein (GFP), Clontech; [γ-32P]ATP, New England Nuclear; MDY-64 from Invitrogen Molecular Probes; ‘Complete mini’ protease mix, Roche.



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